Fig. 8

Neural stem cells derived from cells lacking Hmgn1 or Hmgn2 show loss of NSC identity. Immunostaining for a nestin and b βIII tubulin in NSCs derived from parental P19 cells, Hmgn1 and Hmgn2 knockout lines. Scale bar indicates 50 µm. c Relative mRNA expression in NSCs derived from parental P19 cells, CON-a cells, and Hmgn1 and Hmgn2 knockout lines. Expression in parental NSCs that were induced to differentiate down the neuronal lineage by removal of growth factors is also shown. Expression is normalised to that of Gpi1, and is plotted relative to that in parental NSCs. Error bars represent the sd from technical qRT-PCR triplicates. Data are representative of two independent NSC derivation experiments