Fig. 7
Global reduction of histone acetylation in Hmgn-knockout cells. Western blotting for the detection of H3K4me3, H3K9ac and H3K27ac. Acid histone extracts were prepared in duplicate from two different cell passages of each cell line and separated by SDS-PAGE. Western blotting for each histone modification and sample set was performed twice or three times. Representative images are displayed (left). The H3 protein is indicated by an arrow, and additional bands on this blot are the results of non-specific antibody interactions. The intensity for each modification was normalised to that of H3, and the average and s.e.m. is plotted relative to that in parental cells (*p < 0.05, **p < 0.01)