Fig. 6

Suz12 is required for RA-induced Hox upregulation. a Suz12 mRNA in cranial neural tissue of RA-induced mouse NTDs was measured by RT-qPCR. Actb was used as a loading control. Data are shown as the mean (SD; n = 4). *P < 0.05. b Suz12 mRNA in mouse ESCs treated with RA was measured by RT-qPCR. Actb was used as a loading control. Data are shown as the mean (SD; n = 3). *P < 0.05. c H3K27me3 level after upon RA treatment was measured by Western blotting, respectively, by knockdown of Suz12. F9 cells were knockdown of Suz12 for 24 h, and then treated with RA. H3 was used as a loading control. d Knockdown of Suz12 affected mRNA level of Hox genes in RA-induced F9 cells. F9 cells were knockdown of Suz12 for 24 h. Then, after 24 h of RA treatment, cells were collected and analyzed. Data were shown as mean ± SD (n = 3). *P < 0.05. e ChIP assays of H3K27me3 were performed using mouse F9 cells after siSuz12 transfection. Mouse IgG was used as control. Enrichment of Hox gene promoters was measured by qPCR