Fig. 2

Characterization of differential distal THSSs during HD astrocyte differentiation. a Genome-wide distribution of differential THSSs between HD and WT cells across differentiation identified from ATAC-seq non-nucleosomal fragments. Both distal vs. proximal (top) and intergenic vs. intragenic distributions (bottom) are reported. THSSs located ± 500 nt from a TSS are considered proximal. b Heatmap depicting differential enrichment of distal ATAC-seq THSSs at each stage of differentiation. The red color represents HD enrichment and the blue color indicates HD depletion. c Genome-wide distributions of distal ATAC-seq THSSs from HD and WT cells at each timepoint. d, e TF motifs identified from distal ATAC-seq peaks that were enriched in HD (D) and WT (E) cells. Only TF motifs with significant enrichment at least at one stage were included (p < 0.05). f Venn diagram showing overlap of differential distal ATAC-seq THSSs and Putative Active Brain Enhancers (PABEs) previously published [43]. Purple denotes ATAC-seq peaks and green indicates macaque brain enhancers. g Genome-wide distribution of differential enhancer THSSs across HD astrocyte differentiation. h Heatmap depicting differential enrichment of distal ATAC-seq THSSs at PABEs during differentiation. The red color represents HD enrichment and the blue color indicates HD depletion