Fig. 7From: Folate deficiency induced H2A ubiquitination to lead to downregulated expression of genes involved in neural tube defectsa Elisa assay detected folate content in brain tissue of normal fetus and fetus with NTDs. b Detection of H2AK119ub1 in brain tissues from normal fetus and low-folate NTDs with low folate maternal serum. H2A was used as a loading control. Data are mean ± SD. (n = 3),*P < 0.05, by Student’s t test. c Quantification of the western blotting H2AK119ub1 signal intensity shown in scatter plot. Column normals: n = 8; Column NTDs: n = 8; *p < 0.05, by Student’s t test. NTDs vs. normal brains. d Pearson R- and P value for normalized H2AK119ub1 levels versus low folate content in low-folate NTDs fetuses (n = 8). Data are presented as mean ± SEM. ***P = 0.002. e The mRNA expression of NTC-related genes CDX2, NESTIN, PAX6 and GATA4 in the NTDs fetuses brain, determined by Nanostring. Data are mean ± SD (n = 10),*P < 0.05, by Student’s t test. f Pearson’s correlation analysis between H2AK119ub1 expression and NTC-related genes CDX2, NESTIN, PAX6 and GATA4Back to article page