Fig. 1

A three-step process to perform methylome dissection using eigen-pCSM loci. a In the first step, bipolar 4-CG segments are identified and a nonparametric Bayesian clustering algorithm is used for the determination of pCSM loci. b In the second step, co-methylation analysis is performed by k-means clustering coupled with WGCNA analysis. In each co-methylation module, PCA analysis is performed to pick the eigen-pCSM loci as a representative for the whole module. c In the third step, methylome dissection is performed by nonnegative matrix factorization (NMF), where matrix N stands for the raw methylation profile and is decomposed into two matrices, W and H. Matrix W represents the methylation profile of cell components, and matrix H represents the proportion of cell components