TY - JOUR AU - Nechin, Julianna AU - Tunstall, Emma AU - Raymond, Naideline AU - Hamagami, Nicole AU - Pathmanabhan, Chris AU - Forestier, Samantha AU - Davis, Tamara L. PY - 2019 DA - 2019/10/17 TI - Hemimethylation of CpG dyads is characteristic of secondary DMRs associated with imprinted loci and correlates with 5-hydroxymethylcytosine at paternally methylated sequences JO - Epigenetics & Chromatin SP - 64 VL - 12 IS - 1 AB - In mammals, the regulation of imprinted genes is controlled by differential methylation at imprinting control regions which acquire parent of origin-specific methylation patterns during gametogenesis and retain differences in allelic methylation status throughout fertilization and subsequent somatic cell divisions. In addition, many imprinted genes acquire differential methylation during post-implantation development; these secondary differentially methylated regions appear necessary to maintain the imprinted expression state of individual genes. Despite the requirement for both types of differentially methylated sequence elements to achieve proper expression across imprinting clusters, methylation patterns are more labile at secondary differentially methylated regions. To understand the nature of this variability, we analyzed CpG dyad methylation patterns at both paternally and maternally methylated imprinted loci within multiple imprinting clusters. SN - 1756-8935 UR - https://doi.org/10.1186/s13072-019-0309-2 DO - 10.1186/s13072-019-0309-2 ID - Nechin2019 ER -