Fig. 2

Differential methylation with aging is anti-correlated with expression changes in gene body and enhancer regions. a Volcano plots of mRNA differential expression with aging (multiple linear regression, FDR < 0.05, |FC| > 1.25, n = 6/group) in males and females. b Venn diagrams of the overlap of upregulated and downregulated differentially expressed genes between males and females. Correlation between age-DMRs mapped to promoters (c, f), gene body (D,G) or enhancer regions (e, h) and gene expression fold change (O/Y) in statistically significant (blue) and non-statistically significant genes (red) in females (c–e) and males (f–h)