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Table 1 Comparison of the levels of repression at the FLO loci, VIIL telomere and the mating type loci

From: Histone chaperones and the Rrm3p helicase regulate flocculation in S. cerevisiae

Strain Flocculation Increase in FLO gene expression Silencing at the VIIL telomere (%FOAR cells) Silencing at the HML/HMR mating type loci
BY4742 No n/a 66% (25) No loss [34]
sir3∆ No < 1x < 0.1% Loss [34]
cac1∆ No 1–2x 5% (16) No loss [25, 34], transient loss [19]
rrm3∆ No 1–2x 10% (16) No loss (Additional File 1)
asf1∆ No 1–2x 9% (25) No loss [34] transient loss [19]
hir1∆ No 1–2x 53% (25) No loss [25]
cac1∆asf1∆ Yes > 5x < 1% (25) Loss [25, 34]
cac1∆hir1∆ Yes > 5x < 1% (25) Loss [25]
cac1∆rrm3∆ Yes > 5x < 0.1% (16) Loss (Additional File 1)
asf1∆rrm3∆ Yes > 5x < 0.1% (16) Loss (Additional File 1)
hir1∆rrm3∆ No > 5x 11% (16) No loss (Additional File 1)
orc5-1 Yes > 5x < 1% (25) Loss [47]
sas2∆ No n/a 4% (25) No loss (Additional File 1)
tof1∆ No n/a 26% (16) n/a
mcm5-461 No n/a 4% (25) n/a
bob1-1cdc7∆ No n/a 3% (25) n/a
cac1∆tof1∆ No n/a n/a n/a
cac1∆sas2∆ No n/a n/a n/a
tof1∆rrm3∆ No n/a n/a n/a
hda1∆ No n/a n/a n/a
hst1∆ No n/a n/a n/a
rpd3∆ No n/a n/a n/a
  1. Data from this study and [4, 16, 19, 25, 34] were used to compare the magnitude of de-repression in different mutants. The de-repression of FLO genes is based on the data in Fig. 2 and expressed as the average fold increase in the expression of FLO1, FLO5, FLO9, FLO10, FLO11 in the mutants relative to BY4742 in both exponentially growing and saturated cultures. The sub-telomeric gene repression is measured by the routine Telomere Position Effect (TPE) assay [46] and is displayed as per cent of 5-Floro-Orotic-Resistant (FOAR) cells, which carry URA3 adjacent to the VIIL telomere. The repression of mating type loci is measured by the expression of a GFP reporter inserted in HMR [25, 34]; by the CRASH assay, which measure transient expression from the HML locus; or by measurement of the mating efficiency (this study) “No loss” means less than 0.01% GFP+ cells and “loss” means more than 10% GFP+ cells. The “transient” and “strong” are used exactly as in [19]
  2. n/a not available in any of the listed sources