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Table 2 List of antibodies used in the present study

From: TH2BS11ph histone mark is enriched in the unsynapsed axes of the XY body and predominantly associates with H3K4me3-containing genomic regions in mammalian spermatocytes

Reagents

Antibodies

Host

Company name

Cat number

Application

Scp3

Mouse

Abcam

ab97672

IF

γH2AX

Mouse

Upstate (Millipore)

05-636

IF, ChIP, WB

H2BS14ph

Rabbit

Scbt

sc31671

WB

pATM

Mouse

Upstate (Millipore)

05-740

IF

ATR

Mouse

Abcam

ab54793

IF

H3K4me3

Mouse

Abcam

ab12209

ChIP, WB

TH2B

Rabbit

Generated in house

 

IF, WB

Rad51

Mouse

Abcam

ab1837

IF

Spo11

Goat

Santa Cruz

sc22476 

IF

  1. IF immunofluorescence, ChIP chromatin immunoprecipitation, WB western blotting