Fig. 7
Determination of interacting protein partners of TH2BS11ph-containing mononucleosomes in rat testicular cells. a Silver-stained image of the TH2BS11ph ChIP-Input lane refers to 5% input; IgG lane refers to non-specific IgG control; TH2BS11ph refers to TH2BS11ph IP lane. b List of interacting proteins of TH2BS11ph-containing mononucleosomes as determined by mass spectrometry in rat testicular cells. The associated proteins are classified into three different categories based on their known functions: XY body, Transcription and other important proteins. The first row is the gene/protein name; the second row refers to the sum intensity that refers to the peak intensity values for all the peptides matched to a particular protein. The proteins highlighted in red refer to common proteins identified between TH2BS11ph IP and γH2AX IP (as reported by Broering et al. [48]). c Model of TH2BS11ph-containing nucleosomes showing the association with XY body-related histone marks. TH2BS11ph could function along with γH2AX and H3K4me3 histone marks to mediate DNA repair in the XY body. d Model of TH2BS11ph-containing nucleosomes showing the association of this histone mark with transcription start sites. TH2BS11P could associate with H3K4me3, H2AZ and transcription-associated H4 acetylated marks to mediate transcription in pachytene cells. TH2BS11ph could function in association with specific repertoire of histone marks to mediate chromatin-templated events like DNA repair in the XY body or TSS activation