Fig. 6

Validation of the ChIP sequencing dataset of TH2BS11ph in mouse P20 testicular cells. a Genomic regions used for designing primers required to confirm the ChIP-sequencing dataset by ChIP-PCR studies. Every top lane in all the panels from (i)–(vii) is TH2BS11ph IP, bottom panel-TH2B IP. b Validation of TH2BS11ph genome-wide occupancy data using ChIP-PCR technique using TH2B and TH2BS11ph antibodies. ChIP-PCR shows the enrichment of TH2BS11ph histone mark at the selected genomic regions associated with Chromosome X (2 regions), Chromosome Y and autosomes (2 regions) over TH2B IP and input control (i–v). A specific region in chromosome 15 and chromosome 1 were used as negative controls for this study (vi and vii). The fold enrichment values of TH2B and TH2BS11ph IPs were plotted against input control. ChIP-PCR experiments were done for three biological replicates including technical duplicates for a single biological replicate. The data are plotted in terms of mean ± SD, ***P ≤ 0.0005, **P ≤ 0.005, *P ≤ 0.05 (t-test)