Fig. 3

TH2BS11ph histone mark is predominantly associated with genomic regions containing the histone mark H3K4me3. TH2BS11ph-containing mononucleosomes associate with sex body-specific histone mark γH2AX-Mononucleosome IP studies determining the coexistence of histone marks TH2BS11ph with γH2AX, a γH2AX is associated with TH2BS11ph-containing mononucleosomes. IP was carried out using the anti-TH2BS11ph antibody where the TH2BS11ph, γH2AX and TH2B were probed by western blotting. b TH2BS11ph is associated with γH2AX-containing mononucleosomes. IP was carried out with the γH2AX antibody, and scored for TH2BS11ph, γH2AX, and TH2B by western blotting. The antibodies used for western blotting are indicated to the side of the blot. Ponceau stained blots have been given for reference. Data information in a, b The numbers represent molecular weight in kilodaltons. The first lane in all the blots represents the input fraction, 2nd lane IgG elute fraction and 3rd lane immunoprecipitation with the mentioned antibodies. c Workflow of ChIP sequencing analysis for TH2BS11ph histone mark carried out in mouse P20 testicular cells. d Chromosome wise distribution of TH2BS11ph peaks across chromosomes of mouse-majority of the TH2BS11ph peaks were found in chromosomes X and Y. e Distribution of TH2BS11ph and TH2B reads across the X-chromosome. For both TH2BS11ph and TH2B sections, the upper track represents the peaks, whereas the bottom track represents the read distributions as observed in IGV Genome Browser. TH2B ChIP sequencing data were taken from Gene Expression Omnibus (GSE45915) [12]