Fig. 1

SPT16 binding at macrophage distal regulatory regions. a Genomic distribution of SPT16 peaks in BMDM. b SPT16, RNA Pol II and indicated histone modifications profiles at the summit of intergenic SPT16 peaks ± 5 kb. H3K27ac and H3K4me1 ChIP-seq were retrieved from ENCODE. Regions were ranked based on decreasing SPT16 signals. c (Up) Average MNase and ATAC profiles at the summit of intergenic SPT16 peaks ± 2 kb. (Bottom) Overlap between PU.1 and intergenic SPT16 peaks (left). Average PU.1 (middle) and C/EBPα (right) profiles at the summit of intergenic SPT16 peaks ± 2 kb. d Representative examples for SPT16 occupancy along with PU.1, C/EBPα and ATAC-seq profiles at distal regulatory regions. Regions corresponding to intergenic SPT16 peaks are highlighted. e RNA Pol II, GRO-seq and indicated chromatin marks profiles at bodies of genes associated with intergenic SPT16 peaks. Genes were clustered into two major categories based on decreasing RNA Pol II levels. f Nucleosome occupancy and SPT16 profile at the TSS of cluster1 genes