Fig. 5

Summary of H3K27me3 dynamics in PGCs. a Schematic of H3K27me3 staining in representative female PGC nuclei of different Prdm14 genotypes. b PGC numbers and H3K27me3 changes during PGC migration along the hindgut at E9.5. PGCs are most frequently seen in quadrant 2 (Q2), and their number is strongly reduced in Prdm14^−/− embryos. Global H3K27me3 levels are upregulated in most PGCs in Prdm14^+/+ embryos throughout the hindgut, while it is less frequent in Prdm14^+/− and rarely seen in Prdm14^−/− embryos. The accumulation of H3K27me3 on the inactive X-chromosome (X-spot) is progressively lost during migration in both Prdm14^+/+ and Prdm14^+/− PGCs, but mostly maintained in Prdm14^−/− PGCs. c PGC numbers and H3K27me3 changes in E9.5 embryos with different developmental progression (somite number). PGC numbers and global H3K27me3 levels increase with developmental progression in Prdm14^+/+ and Prdm14^+/− (reduced upregulation), but not in Prdm14^−/− embryos. The X-chromosomal H3K27me3 spot is lost in both Prdm14^+/+ and Prdm14^+/− PGCs independently of developmental progression, while Prdm14^−/− PGCs maintain the X-spot at all stages