Fig. 1

Prdm14^−/− E9.5 embryos have reduced PGC numbers with altered distribution along the hindgut. a Numbers of AP2γ-positive PGCs per embryo across Prdm14 genotypes. Means and 95% confidence intervals are shown as error bars. Kruskal–Wallis test was used for statistical comparison (p < 0.001). b Linear regression of AP2γ-positive PGC numbers versus developmental stage (somite number). R-square values for Prdm14^{+/+, +/−} and ^−/− embryos were 0.659, 0.514 and 0.001, respectively. c Image of a E9.5 mouse embryo with PGCs (drawn green dots) migrating along the hindgut divided into four quadrants (Q1–Q4). Scale bar: 250 μm. d Distribution of PGCs along the hindgut across Prdm14 genotypes