Fig. 6
SSTR5-AS1 recruits TET1 to its target gene E-cadherin to increase its expression through increasing 5hmC levels at the promoter region of E-cadherin. a Phase-contrast micrographs of AMC-HN-8 cells treated with TGF-β1 for 21d. b Relative mRNA expression levels of EMT markers in AMC-HN-8 cells treated with TGF-β1. *Compared with untreated cells, P < 0.05. c Relative expression level of SSTR5-AS1 in AMC-HN-8 cells treated with TGF-β1. *Compared with untreated cells, P < 0.05. d Relative mRNA expression levels of EMT markers in SSTR5-AS1-transfected AMC-HN-8 cells. *P < 0.05. e Relative protein expression levels of EMT markers in SSTR5-AS1-transfected AMC-HN-8 cells. f The 5hmC enrichment at the promoter region of E-cadherin was determined by hMeDIP-qPCR method. *P < 0.05. g Overexpression of SSTR5-AS1 increased the enrichment of TET1 at the promoter region of E-cadherin detected by ChIP assay. *P < 0.05. h RIP assay showed the binding of SSTR5-AS1 to TET1 in SSTR5-AS1-transfected AMC-HN-8 cells. *P < 0.05