Fig. 6
From: Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors
Sp1ΔDBD/ΔDBD cells contain an additional megakaryocyte cluster as compared to WT cells. a t-SNE visualisation of combined WT and Sp1ΔDBD/ΔDBD populations. Each dot represents a single cell. In the left panel, dots are coloured to represent the genotype as indicated. In the right panel, dots are coloured according to the cell identity as indicated. b The expression level of selected differentially expressed genes was superimposed onto the t-SNE plots shown in Fig. 6a. Gene names are indicated to the right of the panel. c Venn diagrams representing the overlap between Sp1 ChIP-seq targets in ESC (light green circles) or Flk1+ cells (dark green circles) with up-regulated genes (red circles) or down-regulated genes (blue circles) determined from single-cell differential gene expression analysis. The number of genes for each group is indicated