Fig. 4
From: Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors
Deregulated gene expression in Sp1ΔDBD/ΔDBD and Sp3−/− cell populations. a Hierarchical clustering of differentially regulated genes between WT, Sp1ΔDBD/ΔDBD and Sp3−/− cell populations from each stage of the differentiation series according to the row Z score. Data were separated into 13 clusters, and the number of the cluster is shown to the left of the heat map. Representative genes from each cluster are shown to the right of the heat map. b Box plots representing the Z score of gene expression of each individual cluster from (a). c Heat map representing the grouping analysis of differentially regulated genes for Sp1ΔDBD/ΔDBD relative to WT and Sp3−/− relative to WT in ESC. The coloured sidebar indicates the 8 clusters assigned according to the changes in gene expression. GO terms for selected groups are shown to the left of the heat map. The table beneath shows the number of deregulated genes in each group and the number of Sp1 and Sp3 target genes within each cluster. d Heat map representing the grouping analysis of differentially regulated genes for Sp1ΔDBD/ΔDBD relative to WT in Flk1+ cells, and Sp3−/− relative to WT in Flk1+ cells. The coloured sidebar indicates the 8 clusters assigned according to the changes in gene expression. GO terms for selected groups are shown to the left of the heat map. The table beneath shows the number of deregulated genes in each group and the number of Sp1 and Sp3 target genes within each cluster