Fig. 3
From: Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors
Sp3−/− ES cells exhibit a defect in hematopoietic differentiation. a Genome browser screenshots depicting examples of shared and unique binding sites. Top panel: Sp1 is an example of an Sp1/Sp3 shared promoter binding site; middle panel: Zswim6 is an example of an intragenic Sp1 specific peak (highlighted by the red box); bottom panel: Yod1 is an example of an Sp3 specific promoter peak (highlighted by the blue box). b Flk1 FACS staining on WT and Sp3−/− cells at day 3.25 of EB differentiation (n = 5, **indicates p < 0.01). c Kit FACS staining of WT and Sp3−/− cells at day 2 of blast culture (n = 4, *indicates p < 0.05). d Percentage cell populations from WT and Sp3−/− cells at day 2 of blast culture (n = 4, n.s. indicates not statistically significant). e Percentage of macrophage-releasing EB in a macrophage release assay comparing WT and Sp3−/− cells (n = 4, *indicates p < 0.05). f Number of primitive erythroid colonies in an EryP colony formation assay comparing WT and Sp3−/− cells (n = 4, *indicates p < 0.05)