Fig. 2

Sp3 partially compensates for the absence of Sp1 in cells with disrupted Sp1 binding. a Average profiles of Sp1 ChIP enrichment in ESC (left panel) and Flk1+ cells for WT and CRISPR clones. b Heat maps showing a ranking of normalised tag counts from Sp1 ChIP-seq in A17Lox WT ES cells. Ranked alongside are tag counts from the Sp3 ChIP-Seq in the same cells and motifs for CTCF, NFY, YY1, ESRRB, NANOG, OCT4 and SOX2. Heat maps (public datasets, see Additional file 1: Table 1) showing ChIP-seq enrichment for CTCF, NFY, YY1, ESRRB, NANOG, OCT4 and SOX2. The histone modifications H3K9Ac, H3K27me3, H3K4me3 and H3K27Ac are shown alongside. c Heat maps showing fold difference in ChIP-seq enrichment between Sp1 and Sp3 ChIP in E14 WT ES cells. Ranked along the same coordinates are Sp1 and Sp3 ChIP tag counts in Sp1^ΔDBD/ΔDBD and Sp1^−/−  cells. Peaks were classed as specific if they showed equal to or more than twofold change in tag count difference between the Sp1 and Sp3 ChIPs. The specific and shared groups are indicated by coloured bars alongside (red indicates Sp1 specific peaks and blue indicates Sp3 specific peaks), and the number of peaks within each group is shown. d Heat maps showing fold difference in ChIP-seq tag count enrichment between Sp1 and Sp3 ChIP peaks in Flk1+ cells. Ranked along the same coordinates are Sp1 and Sp3 ChIP in Sp1^ΔDBD/ΔDBD  cells. Peaks were classed as specific if they showed more than twofold change in tag count difference between the Sp1 and Sp3 ChIPs. The specific and shared groups are indicated by coloured bars alongside (red indicates Sp1 specific peaks and blue indicates Sp3 specific peaks), and the number of peaks within each group is shown. e Zoomed-in heat map of the 1975 Sp1 specific sites in ESC shown in Fig. 2c. Right-hand panels show the Sp3 ChIP-seq enrichment ranked according to the changes in Sp3 occupancy at these sites in Sp1^ΔDBD/ΔDBD and Sp1^−/−  cells. The blue bar indicates peaks which are increased in the mutant cell lines compared to WT, and the green bar indicates peaks which are reduced at least twofold in the mutant cell lines compared to WT. f Zoomed-in heat map of 873 Sp1 specific sites in Flk1+ cells shown in Fig. 2d. Right-hand panel shows the Sp3 ChIP-seq enrichment ranked according to the changes in Sp3 occupancy at these sites. The blue bar indicates peaks which are increased in the mutant cell lines compared to WT, and the green bar indicates peaks which are reduced at least twofold in the mutant cell lines compared to WT. g Bar graphs indicating numbers of motifs within the 1975 Sp1 specific peaks in ESC. Separate graphs show all peaks, distal peaks and promoter peaks. The number of peaks is indicated above each bar. h Bar graphs indicating numbers of motifs within the 873 Sp1 specific peaks in Flk1+ cells. Separate graphs show all peaks, distal peaks and promoter peaks. The number of peaks is indicated above each bar