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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Phosphorylation of TET2 by AMPK is indispensable in myogenic differentiation

Fig. 5

TET2 regulates Pax7 expression through a putative enhancer located in intron 7 of Pax7. a Increased DNA methylation at a potential intragenic enhancer of Pax7. Normalized MeDIP-Seq and RNA-Seq tag densities in myoblasts (MB) and myotubes (MT) are shown. Peaks of H3K4me3 (MB: GSM628005; MT: GSM628006), H3K4me1 (MB: GSM1197187; MT: GSM1197187) and H3K27ac (MB: GSM921131; MT: GSM921133) were downloaded through cistrome DB. The locations of the Pax7 enhancer and sgRNA targeting sites for enhancer knockout are indicated. The myoblast-specific hyper-DMR in the intron 7 of Pax7 is indicated in blue. Results from two independent AMPK-KO lines are shown. b PCR screening for CRISPR/Cas9-mediated deletion of Pax7 intragenic enhancer in C2C12 cells. The locations of the PCR primers used for genotyping are displayed in panel (a). c The expression of Pax7 in C2C12 clones with deletion of Pax7 intragenic enhancer was examined by RT-qPCR. Csnk2a was used for normalization. Data are presented as mean ± SD from three independent experiments performed in triplicates. **p < 0.01

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