Fig. 4
From: Phosphorylation of TET2 by AMPK is indispensable in myogenic differentiation
Genome-wide DNA methylation changes in AMPK knockout C2C12 cells. a Levels of 5mC (left) and 5hmC (right) in wild-type (WT) and AMPK knockout (KO) C2C12 cells at myoblast (d0) and myotube (d8) stages were detected by mass spectrometry. Two independent AMPK-KO lines were used. Data are presented as mean ± SD of triplicates. **p < 0.01. b Normalized methylated DNA immunoprecipitation sequencing (MeDIP-Seq) tag densities at genic and enhancer regions in wild-type (WT) and AMPK-/- C2C12 cells. Enhancers were defined according to their histone modification patterns [25]. 5mC enrichments from − 2.5 kb to + 2.5 kb relative to gene bodies or the centers of enhancers are shown. Two independent AMPK-KO lines were used. c The distribution of differentially methylated regions (DMRs) in AMPK-KO relative to wild-type C2C12 cells at myoblast (d0) and myotube (d8) stages across the genome. The number of hyper- or hypomethylated DMRs is shown on top of each bar