Human monocyte-to-macrophage differentiation involves highly localized gain and loss of DNA methylation at transcription factor binding sites

Table 1 Summary of the primary results

Differentiation	DMCs	Gain	Loss
	5780	4283	1497

Activation	DMCs	Gain	Loss
M (LPS/IFNγ)	69	4	65
M (IL-4)	6	3	3
M (oxLDL)	4	0	4
M (acLDL)	3	0	3

Macrophage-specific	DMCs
	8

DNA methylation in all subsets was measured using the Illumina 450k array, and an epigenome-wide analysis was performed using a linear mixed model with donor as random effect
Shown are the amount of differentially methylated CpGs (DMCs) during differentiation and activation based on partial t-statistic and split by gain and loss of methylation (P_FDR < 0.05, mean squares > 0.0025)

ISSN: 1756-8935