Fig. 4

TET1 KD reduces cell proliferation of human T-ALL. Human T-ALL cells (CCRF-CEM) were compared before (SCR) and upon TET1 KD (TET1sh). RT-qPCR of a MYC and TET1, b TET2 and TET3. RT-qPCR data were normalized to RPL13a. c Growth curve comparing viable cell counts. d Flow cytometric cell cycle analysis based on propidium iodide (PI) staining. The cell cycle distribution (G1, S, and G2/M) is displayed in percent. e Flow cytometric analysis of apoptosis based on Annexin V/PI staining. Flow cytometry profile of Annexin V staining (X axis) and PI (Y axis) is shown for representative samples. The lower right quadrant indicates the percentage of early apoptotic cells in each condition; the upper right quadrant indicates the percentage of late apoptotic cells; the upper left quadrant indicates the percentage of necrotic cells. Apoptotic cells (Annexin V-positive cells) are displayed as the percentage of gated cells. Error bars represent mean ± SEM; n = 3; two-tailed Student’s t test: *P < 0.05; **P < 0.01; ***P < 0.001