Fig. 8From: Ovarian dysfunction following prenatal exposure to an insecticide, chlordecone, associates with altered epigenetic featuresThe decrease in histone H3K4me3 and H4 acetylation levels in adult ovaries following gestational CD exposure. Exposure to CD affects histone H3K4me3 and H4Ac levels in adult ovaries. a Representative images of control (top) or treated oocyte (bottom) immunostained by MSY2 (oocyte marker, green) or H3K4me3 (red) (40X magnification). b Quantitative analysis of H3K4me3 in oocytes and the surrounding granulosa cells, n = 4 for each group, *p < 0.05, **p < 0.01, t test. c Representative images of antral follicles immunostained for acetylated histone 4 (red). Note that in control samples homogenous nuclear staining is observed, while in treated samples clusters of bright dots are visible. d Quantitative analysis of H4 acetylation in granulosa cells and oocytes of control and CD-treated samples, n = 4 for each group, *p < 0.05, t testBack to article page