Fig. 4

Meiosis defects are associated with altered H3K27me3 occupancy. a Surface spreads from E15.5 control (top row) and CD-exposed (bottom row) ovaries were immunostained with anti-H3K27me3 (green) and anti-SYCP3 (red) antibodies, b expression of H3K27me3 in somatic cells (63X magnification). The Barr body is shown with an arrow. Quantitative analysis of the anti-H3K27me3 intensity in c meiotic cells and d somatic cells. The analysis was performed on at least 20 oocytes from four different animals for each group, n = 4 for each group, *p < 0.05, **p < 0.01, ***p < 0.001, t test. e The gene expression level of Ezh2 was analyzed by RT-qPCR using RNA from E15.5 ovaries from control and CD-treated animals, *p < 0.05, t test