Fig. 2

Critical meiotic step is perturbed in embryonic ovaries exposed to CD. a Surface spreads from E15.5 control (top row) and CD-treated ovaries (bottom row) were immunostained with anti-DMC1 (green) and anti-SYCP3 (red) antibodies (63X magnification). b Surface spreads from E17.5 control and CD-treated ovaries. c, d Quantitative analysis of the number of DMC1 foci per cell performed on at least twenty of E.15 and 17.5 oocytes from four replicates from different litters, n = 4 for each group, **p < 0.01, t test. e The expression levels of genes involved in DSBs repair were analyzed by RT-qPCR using RNA from E15.5 ovaries from control and CD-treated animals, *p < 0.05, t test