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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Ovarian dysfunction following prenatal exposure to an insecticide, chlordecone, associates with altered epigenetic features

Fig. 1

The gestational exposure to CD leads to oxidative stress. Gestational exposure to CD increases the accumulation of 8-oxo-G in fetal oocytes. a Representative images of E15.5 oocytes immunostained with antibodies against 8-oxo-G (red), or SYCP3 (green) in structurally preserved nuclei are shown (63X magnification). Given the nuclear 3D structure, we used z-stack image processing technique as described in Methods section, followed by image deconvolution. In the merged image that represents the top view (X/Y axis) of the image, the section views of the X/Z axes (top) and Y/Z axes (right) are also shown. b Quantitative analysis of 8-oxo-G staining in E15.5 samples which represents the mean-averaged intensities of 21 individual image planes that were calculated separately in cytoplasm and nucleus. The data were expressed as normalized fluorescence compared to control ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, t test. c The normalized mRNA expression level of Nudt1, *p < 0.05, t test. The gene expression was normalized to the expression of germ cell marker, Dazl gene

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