Fig. 2From: Dam mutants provide improved sensitivity and spatial resolution for profiling transcription factor bindingDamID-seq protocol. DamID-seq protocol. DpnI digestion produces blunt ends at all methylated sites, onto which a forked adapter containing an Illumina sequencing site is ligated. A second sequencing adapter is introduced randomly throughout the genome by a transposase (Nextera tagmentation). Fragments containing both adapters are amplified and directly sequenced. Following alignment, all reads originating at a GATC are summed to give an estimate of amount of methylationBack to article page