Fig. 7

Levels of histone H4 and histone H4 acetylation are altered in histone H4 mutants. Cells of the indicated genotypes were grown to mid-log phase and incubated in the absence or presence of 15 µM CPT. Western blotting of whole-cell extracts was performed using the anti-H4 and anti-acetyl (K5/8/12/16) histone H4 antibody. a, b Quantification of H4 protein amounts (in a) and total levels of H4 acetylation (in b) was performed using Image J software. c The level of H4 acetylation was also normalized to the total H4 level in each cell extract. Error bars correspond to standard errors of mean (SEM) obtained from three independent experiments