Fig. 3

Flow cytometry analysis of NuA4 mutants. The exponentially growing (Log) cells of the indicated genotypes were incubated with 15 mM HU for 5 h at 25 °C to stall cell cycle progression at the beginning of S-phase (HU). Cells were then released into YES medium supplemented with 15 µM CPT for the indicated time and subjected to cell cycle analysis using a FACS LSRFortessa cell analyzer