Fig. 6

a The mRNA expression of CUL4B and Hox genes in the brain of NTD fetuses, determined by NanoString. Data are mean ± SD (n = 10),*P < 0.05, by Student’s t test. b Detection of H2AK119ub1 in brain tissues from human anencephaly and normal cases by western blotting. Total histone GAPDH was used as a loading control. Data are mean ± SD (n = 3),*P < 0.05, by Student’s t test. c Pearson’s correlation analysis between H2AK119ub1 expression and HOXA1, HOXA9, and HOXA10 expression