Fig. 4

a NT2 cells were transfected overexpression of CUL4B. Thirty-six hours after transfection, RORE luciferase activity was measured after treatments with RA (1uM) for 12 h. Data are mean ± SD (n = 3). b NT2 cells were transfected with the siRNA-CUL4B. Thirty-six hours after transfection, RORE luciferase activity was measured after treatments with RA (1uM) for 12 h. Data are mean ± SD (n = 3). c NT2 cells were transfected with the increasing amounts of CUL4B. Thirty-six hours after transfection, RORE luciferase activity was measured after treatments with RA (1uM) for 12 h. Data are mean ± SD (n = 3). d Knockdown of RORγ affected mRNA level of Hox genes in RA-induced NT2 cells. NT2 cells were knockdown of ROR for 24 h. Then, after 24 h of RA treatment, cells were collected and analyzed. Data were shown as mean ± SD (n = 3). *P < 0.05. e Quantitative ChIP analysis was performed to analyze the effect of RORγ loss on the RA-induced changes in H2AK119ub1 levels at the Hox genes. Enrichment of the Hox genes promoter was measured by qPCR. Data were shown as mean ± SD (n = 3). *P < 0.05