Fig. 3

a The whole NT2 cell extract was immunoprecipitated with anti-CUL4B antibody and normal rabbit IgG antibody, and analyzed by western blot with anti-CUL4B and anti-RORγ antibodies. b Expression level of RORγ was measured by Western blotting after knockdown of CUL4B. c Expression level of H2AK1119ub1 was measured by Western blotting after knockdown of RORγ. d Quantitative RT-PCR analysis showed that CUL4B loss and RORγ had a negative effect on HOX genes in NT2 cells. Data were shown as mean ± SD (n = 3). *P < 0.05. e ChIP assays of H2AK119ub1 were performed using NT2 cells after knockdown of CUL4B and RORγ; IgG was used as control. Enrichment of the HOX genes promoter was measured by qPCR. Data were shown as mean ± SD (n = 3). *P < 0.05