Fig. 2

a Knockdown of CUL4B affected histone H2AK119ub1 modification level in NT2 cells. b Comparison of histone H2AK119ub1 modification level and Cul4b level between normal and RA-treated mouse ESCs by Western blotting. c The colocalization of Cul4b and H2AK119ub1 in the mouse ESCs cells with RA treatment. Direct immunofluorescence analysis was performed. Images were captured by confocal microscope, and the nuclei were stained with DAPI. d ChIP assays of H2AK119ub1 were performed using mouse ESC after RA treatment; mouse IgG was used as control. Enrichment of the Hox genes promoter was measured by qPCR. Data were shown as mean ± SD (n = 3). *P < 0.05. e ChIP assays of H3K27me3 were performed using mouse ESC after RA treatment; mouse IgG was used as control. Enrichment of the Hox genes promoter was measured by qPCR. Data were shown as mean ± SD (n = 3). *P < 0.05. f ChIP assays of Cul4b were performed using mouse ESC after RA treatment; mouse IgG was used as control. Enrichment of the Hox genes promoter was measured by qPCR. Data were shown as mean ± SD (n = 3). *P < 0.05