Fig.Ā 1From: Abnormal level of CUL4B-mediated histone H2A ubiquitination causes disruptive HOX gene expressiona Quantitative RT-PCR analysis showed that CUL4B loss had a positive effect on the RA-induced increases in differentiation genes mRNA levels in NT2 cells. Data were shown as meanāĀ±āSD (nā=ā3). *Pā<ā0.05. b Knockdown of CUL4B affected mRNA level of Hox genes in RA-induced NT2 cells. NT2 cells were knockdown of CUL4B for 24Ā h. Then, after 24Ā h of RA treatment, cells were collected and analyzed. Data were shown as meanāĀ±āSD (nā=ā3). *Pā<ā0.05. c Quantitative ChIP analysis was performed to analyze the effect of CUL4B loss on the RA-induced changes in H2AK119ub1 levels at the Hox genes. Enrichment of the Hox genes promoter was measured by qPCR. Data were shown as meanāĀ±āSD (nā=ā3). *Pā<ā0.05Back to article page