Fig. 4

Sequence composition of regions with variant nucleosomes in KDM5B-depleted ES cells. a Average profile of AT and GC density at nucleosomes and flanking regions in control and KDM5B-depleted ES cells. GC sequence composition is elevated in nucleosome occupied regions relative to flanking regions, while the pattern of AT sequence composition is opposite. Variant b, c + 1 nucleosomes and d, e − 1 nucleosomes exhibit altered b, d GC and c, e AT Sequence composition in KDM5B-depleted ES cells (nucleosome shift distance: 0, 1–9, 10–50, 51–100, 101–150, 151–200 bp). f Schematic of nucleosome shift directionality relative to GC/AT sequence composition