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Fig. 4 | Epigenetics & Chromatin

Fig. 4

From: Targeted in vivo epigenome editing of H3K27me3

Fig. 4

H3K27me3 epigenome editing was highly site specific. a, b Epigenetic modification patterns, sgRNAs (blue bars) and ChIP-qPCR product (black bars) positions around sgRNA target site. ChIP-seq using anti-FLAG antibody (gray) and anti-H3K27me3 (orange) in dCas9-olEzh2(SET) or dCas9-olEzh2 injected embryos are shown. In addition, DNase I-seq (black) [28] and DNA methylation [34] pattern of blastula stage are shown. c MA plot of differential enrichment analysis of ChIP-seq signals of dCas9-olEzh2(SET) injected embryos and dCas9-olEzh2 injected embryos. Each dot shows H3K27me3 peak. The peak with the p value under 0.01 is indicated as red dot. The peaks with the fold change greater than 5 or less than − 5 are indicated as triangles. d Volcano plot of differential enrichment analysis of ChIP-seq signals of dCas9-olEzh2(SET) injected embryos and dCas9-olEzh2 injected embryos. All H3K27me3 peaks are indicated as dots. Only the peak including targeted genomic region is indicated as red dot

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