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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Targeted in vivo epigenome editing of H3K27me3

Fig. 2

H3K27me3 epigenome editing by dCas9-olEzh2 targeting methylated promoters. a, d The epigenetic modification patterns around Tbx16 and Slc41a2a, sgRNAs (blue bars) and ChIP-qPCR product (black bars) positions. H3K27me3 (red) and H3K27ac (blue) ChIP-seq [27], DNase I-seq (black) [28] and DNA methylation enrichment [34] at the blastula stage are shown for comparison. b, c, e, f The results of ChIP-qPCR using anti-FLAG antibody (b, e) and anti-H3K27me3 antibody (c, f). H3K27me3 negative region (K27me3 NC) and H3K27me3 positive region (K27me3 PC) were used for ChIP control (described in Additional file 1: Fig. S2). Light blue, gray and orange bars represent no injection, sgRNAs/dCas9 injection and sgRNAs/dCas9-olEzh2 injection, respectively. (Tukey–Kramer test, *p < 0.1, **p < 0.05, ***p < 0.01, n = 3 biological replicates, error bars are s.d., p values of each comparison are shown only if the p value is under 0.1.)

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