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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Coupling of PARP1-mediated chromatin structural changes to transcriptional RNA polymerase II elongation and cotranscriptional splicing

Fig. 1

PARP1 depletion alters splicing decisions. a Sashimi plots showing changes in the splicing decisions due to PARP1 depletion in RNA-seq genome-wide analyses for AKAP200 and CAPER. b PCR products with exon junction spanning primers validate splicing changes in AKAP200 and CAPER. Agarose gel images and percentage of exons inclusion show the difference in splicing product between non-treated (WT) and PARP1 knockdown (KD) cells. Actin is shown as a PARP1 non-target gene. Additionally, the percentage of each isoform included is calculated as a percentage of all transcripts amplified by PCR set to a 100%

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Epigenetics & Chromatin

ISSN: 1756-8935