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Fig. 7 | Epigenetics & Chromatin

Fig. 7

From: The long non-coding RNA Kcnq1ot1 controls maternal p57 expression in muscle cells by promoting H3K27me3 accumulation to an intragenic MyoD-binding region

Fig. 7

MyoD binds to the maternal p57 intragenic region upon differentiation. a ChIP-qPCR analysis of MyoD binding in undifferentiated (U) and differentiated (D) C2.7 muscle cells using primers for p57 promoter (p57 p) and p57 intragenic region (p57i) (left) and for Myogenin promoter (MyoG p) (right) used as a positive control for MyoD binding during differentiation. b Allele-specific ChIP-qPCR of MyoD binding in polymorphic fibroblasts (C57B/6 female × SD7 male) expressing exogenous MyoD in undifferentiated (U) and differentiated (D) cells, using primers specific for p57 promoter (p57 p), for maternal and paternal p57 intragenic regions (M-p57i and P-p57i, respectively) and for Myogenin promoter (MyoG p). Values were expressed as percentages of Input and error bars indicate SEM of three independent experiments performed, excepted for the part B-left of the figure in which values are the mean ± SEM of four independent experiments. Statistical significance: p value < 0.05 (*) and < 0.01 (**)

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