Fig. 6

EZH2 and H3K27me3 association to the p57 intragenic region decreases after Kcnq1ot1 depletion. C2.7 myoblasts were transfected with Kcnq1ot1 or control siRNAs as in Fig. 1a and analyzed by RT-qPCR (Additional file 5) or ChIP-qPCR. a ChIP-qPCR analysis for control IgG (upper panel) and for EZH2 binding (lower panel) to the p57 promoter (p57 p) and to the p57 intragenic region (p57i). β-Globin promoter (β-Glob p) and Albumin promoter (Alb p) were used as invariant controls, b ChIP-qPCR analysis of H3K27me3 association to the p57 promoter (p57 p), p57 intragenic region (p57i), β-Globin promoter (β-Glob p) used as an invariant control and β-Actin promoter (β-Act p) as a negative control. Values obtained are expressed as percentages of Input chromatin and normalized to those of Albumin promoter, used as an additional invariant control. Results are the mean ± SEM of three independent experiments. Statistical significance p value < 0.05 (*)