Fig. 3

Kcnq1ot1 constitutively interacts with an intragenic region of the maternal p57 allele. a Schematic representation of the p57 gene; black blocks represent exons, black lines introns and gray boxes the regions analyzed for Kcnq1ot1 binding; p57 p indicates the p57 promoter and p57i the p57 intragenic region. b ChOP assays performed in undifferentiated and differentiated C2.7 muscle cells; qPCR analysis of purified DNA performed using primers specific for p57 p, p57i and KvDMR1. Dppa2 and Nap1l4 were used as negative controls. The enrichment with antisense oligonucleotides over scrambled oligonucleotides was plotted in logarithmic scale. Data represent the mean ± SEM of three independent experiments. Statistical significance: p < 0.05 (*). c ChOP assays performed in polymorphic fibroblasts (C57B/6 female × SD7 male); qPCR analysis of purified DNA was performed using allele-specific primers for KvDMR1 (M-KvDMR1: Maternal KvDMR1; P-KvDMR1: Paternal KvDMR1) and p57i (M-p57i: Maternal p57i; P-p57i: Paternal p57i). Values represent one of two independent experiments, and error bars represent the mean ± SEM of each sample analyzed in triplicate