Fig. 8

The Insv:CP190 interaction domain is required for the Insv binding to a subset of sites on polytene chromosomes. A, B Immunostaining of polytene chromosomes from the salivary glands of third-instar ubi-insv⁺ larvae co-stained with antibodies against Insv (A) and CP190 (B); DAPI staining indicates DNA (in blue). The merged image is shown in C. Insv partially localizes to sites of CP190 enrichment. D Wild-type Insv localizes to three sites (arrows 1, 2, and 3) near the distal end of chromosome arm 3 L. In contrast to wild type, Insv that has a deletion of the CP190-interacting domain is not observed at sites 1 and 2, while it is still present at site 3 (E)