Fig. 2

Superose S200 fractionation of HS:C-BEN. a His-Sumo C-BEN (HS:C-BEN) was fractionated on a Superpose S200 column, and the column fractions were then assayed for DNA-binding activity by EMSA using the 32-bp P3 probe. Arrows indicate the two shifts generated by HS:C-BEN. b A₂₈₀ profile of proteins eluted from the Superpose S200 column. Peak 1 has an estimated molecular weight of 190–120 kDa, while the estimated molecular weight of the peak 2 is ~ 60–80 kDa