Fig. 3
Tissue-specific co-methylation regions capture the local co-hydroxymethylation regions. a MHL-based unsupervised clustering of mouse cerebellum, cortex and olfactory bulb using all MHBs. b Comparison of the cluster performance to the brain tissues using the MHL, the average methylation level (AML), the average hydroxymethylation level (AHL) and the average methylation level from BS-seq (AMLBS) metrics in the tissue-specific MHBs. c Lollipop plots showing the 5mC and 5hmC modification of neighboring CpGs of a DNA molecule within a representative olfactory bulb-specific MHB. d Shared MHBs showed bimodal distribution of MHL and low level of 5hmC. AHL: average hydroxymethylation level, AML: average methylation level. e Lollipop plots showing the 5mC and 5hmC modification of neighboring CpGs of a DNA molecule (or a sequencing read) within two representative shared MHBs in olfactory bulb. f Enrichment of tissue-specific MHBs and shared MHBs with low or high MHLs in known genomic features. g The percentages of MHBs with enriched co-modified CpG sites for tissue-specific MHBs and shared MHBs with low or high MHLs in the three tissues. Statistical significance is evaluated by hypergeometric test. ***Represents p value < 0.0001