Fig. 9
From: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
TET1 influences SIN3A and EZH2 binding to osteogenic genes. a BMSC were treated with scramble siRNA (scram), siTET1 or siTET2, cultured under normal growth and then processed for chromatin extraction. Recruitment of SIN3A, HDAC2 or EZH2 molecules to the RUNX2 transcription start site (RUNX2 TSS) was assessed by chromatin immunoprecipitation and normalised to the genomic input control. b Cells were treated as in (a) but enrichment was assessed on the BMP2 transcription start site (BMP2 TSS). c Cells were treated as in (a) but enrichment was assessed on the PPARγ transcription start site (PPAR TSS). d Cells were treated as in (a) but enrichment was assessed on the ADIPSIN transcription start site (ADIPSIN TSS). Data represent mean S.E.M., n = 3 BMSC donors, *p ≤ 0.05, one-way ANOVA with multiple comparison analyses