Fig. 6
From: Specific functions of TET1 and TET2 in regulating mesenchymal cell lineage determination
TET1 and TET2 influence 5hmC on adipogenic genes. BMSC were cultured under normal growth conditions and treated with scramble siRNA or siRNA directed to TET1 (siTET1) or TET2 (siTET2) and genomic DNA purified and immunoprecipitated using an antibody to 5hmC. Recruitment of 5hmC to genomic regions was assessed by the hme-DIP analysis and normalised to the genomic input control. a Relative enrichment of 5hmC on PPARγ transcription start site (TSS) and b ADIPSIN was measured using qPCR. c BMSC were cultured under normal growth (Cont) or adipogenic (Adipo) conditions and then processed for chromatin extraction. Relative enrichment of 5hmC on PPARγ2 transcription start site (TSS), exon and intron regions. d Relative enrichment of 5hmC on ADIPSIN TSS, exon and intron regions. Data represent mean S.E.M., n = 3 BMSC donors, *p ≤ 0.05, one-way ANOVA with multiple comparison analyses