Fig. 3

AutoCUT&RUN reproduces the expected chromatin landscape of H1 and K562 cells. a Scaled Venn diagrams showing the relative amount of the genome that falls within H3K27me3 (gray), H3K4me2 (brown), and H3K27ac (red) domains in H1 cells and K562 cells. Numbers indicate megabases (Mb). b Genome browser tracks showing the overlap of H3K4me2 and H3K27me3 in H1 cells, as well as the expansion of H3K27me3 domains and loss of overlap with H3K4me2 in K562 cells at a representative locus (NODAL). c Heat maps showing the distribution of AutoCUT&RUN profiles of histone modifications in H1 cells centered on the TSSs of genes with a single promoter, oriented left-to-right according to the 5′-to-3′ direction of transcription and rank-ordering according to RNA-seq values (FPKM). d Heat maps showing the distribution of AutoCUT&RUN histone modification profiles on transcriptionally active and repressed promoters in K562 cells. Pearson correlations (r value) between AutoCUT&RUN profiles of individual histone marks around these TSSs and their corresponding RNA-seq values are indicated